Infectious poxvirus vectors have capacity for at least 25 000 base pairs of foreign DNA
GL Smith, B Moss - Gene, 1983 - Elsevier
GL Smith, B Moss
Gene, 1983•ElsevierTo test the capacity of poxviruses for added foreign DNA, a recombinant was constructed
that contains 24 700 bp of bacteriophage λ DNA inserted within the vaccinia virus thymidine
kinase (TK) gene. The recombinant is stable, infectious and replicates in tissue culture at the
same rate and to the same titer as standard vaccinia virus. This size flexibility of the poxvirus
genome and the lack of stringent packaging requirements are useful features for an
infectious eukaryotic cloning vector.
that contains 24 700 bp of bacteriophage λ DNA inserted within the vaccinia virus thymidine
kinase (TK) gene. The recombinant is stable, infectious and replicates in tissue culture at the
same rate and to the same titer as standard vaccinia virus. This size flexibility of the poxvirus
genome and the lack of stringent packaging requirements are useful features for an
infectious eukaryotic cloning vector.
Abstract
To test the capacity of poxviruses for added foreign DNA, a recombinant was constructed that contains 24 700 bp of bacteriophage λ DNA inserted within the vaccinia virus thymidine kinase (TK) gene. The recombinant is stable, infectious and replicates in tissue culture at the same rate and to the same titer as standard vaccinia virus. This size flexibility of the poxvirus genome and the lack of stringent packaging requirements are useful features for an infectious eukaryotic cloning vector.
Elsevier
