Endogenous inhibitors of the dsRNA-dependent eIF-2α protein kinase PKR in normal and ras-transformed cells
LJ Mundschau, DV Faller - Biochimie, 1994 - Elsevier
The serine/threonine kinase PKR is activated by autophosphorylation in response to
nanomolar concentrations of double-stranded RNA and other polyanions. We have
previously shown that expression of an oncogenic ras gene induces an endogenous protein
inhibitor of PKR activation in murine fibroblasts, as measured by a greatly reduced ability of
PKR to autophosphorylate in response to double-stranded RNA in lysates from these ras-
expressing cells. Immunoprecipitation of PKR away from the ras-transformed cell lysate …
nanomolar concentrations of double-stranded RNA and other polyanions. We have
previously shown that expression of an oncogenic ras gene induces an endogenous protein
inhibitor of PKR activation in murine fibroblasts, as measured by a greatly reduced ability of
PKR to autophosphorylate in response to double-stranded RNA in lysates from these ras-
expressing cells. Immunoprecipitation of PKR away from the ras-transformed cell lysate …