Herpes simplex virus-based amplicon vectors have been used for gene transfer into cultured
neurons and the adult CNS. Since constitutive expression of a foreign gene or
overexpression of an endogenous gene may have deleterious effects, the ability to control
temporal expression would be advantageous. To achieve inducible gene expression, we
have incorporated the tetracycline-responsive promoter system into amplicon vectors and
showed, both in vitro and in vivo, that expression can be modulated by tetracycline. Using …