Recombinant human renin produced in different expression systems: biochemical properties and 3D structure
S Mathews, H Döbeli, M Pruschy, R Bosser… - Protein Expression and …, 1996 - Elsevier
Human renin has been expressed in Sf9 and CHO cells using two different gene constructs.
The first construct contained a foreign signal peptide fused directly to the sequence
encoding mature renin, whereas the second construct harbors the sequence for preprorenin.
Prorenin was produced in significantly higher amounts than the mature enzyme expressed
without its propeptide in both expression systems. Both directly expressed mature renin and
proteolytically derived active renin have been purified and cocrystallized with the renin …
The first construct contained a foreign signal peptide fused directly to the sequence
encoding mature renin, whereas the second construct harbors the sequence for preprorenin.
Prorenin was produced in significantly higher amounts than the mature enzyme expressed
without its propeptide in both expression systems. Both directly expressed mature renin and
proteolytically derived active renin have been purified and cocrystallized with the renin …
