DNA content measurement for DNA ploidy and cell cycle analysis
Z Darzynkiewicz, G Juan - Current protocols in cytometry, 1997 - Wiley Online Library
Z Darzynkiewicz, G Juan
Current protocols in cytometry, 1997•Wiley Online LibraryThis unit presents the most common methods of DNA content analysis. The techniques differ
primarily in the mode of cell permeabilization (detergents versus prefixation with alcohol)
and composition of the stain solution. Protocols are given for analysis of fixed cells, tissue
samples, live cells (for subsequent sorting and culture), archival paraffin‐embedded tissue,
and apoptotic cells.
primarily in the mode of cell permeabilization (detergents versus prefixation with alcohol)
and composition of the stain solution. Protocols are given for analysis of fixed cells, tissue
samples, live cells (for subsequent sorting and culture), archival paraffin‐embedded tissue,
and apoptotic cells.
Abstract
This unit presents the most common methods of DNA content analysis. The techniques differ primarily in the mode of cell permeabilization (detergents versus prefixation with alcohol) and composition of the stain solution. Protocols are given for analysis of fixed cells, tissue samples, live cells (for subsequent sorting and culture), archival paraffin‐embedded tissue, and apoptotic cells.
Wiley Online Library