Generation of mature dendritic cells from human blood An improved method with special regard to clinical applicability
N Romani, D Reider, M Heuer, S Ebner… - Journal of …, 1996 - Elsevier
Two methods to generate human dendritic cells from hematopoietic precursor cells in
peripheral blood have recently been published. One approach utilizes the rare CD34+
precursors and GM-CSF plus TNF-α. The other method makes use of the more abundant
CD34− precursor population and GM-CSF plus IL-4. Here we report a method that is based
on the latter approach. However, the GM-CSF and IL-4 treated cells are not stable mature
dendritic cells, eg, the characteristic morphology and nonadherence of dendritic cells is lost …
peripheral blood have recently been published. One approach utilizes the rare CD34+
precursors and GM-CSF plus TNF-α. The other method makes use of the more abundant
CD34− precursor population and GM-CSF plus IL-4. Here we report a method that is based
on the latter approach. However, the GM-CSF and IL-4 treated cells are not stable mature
dendritic cells, eg, the characteristic morphology and nonadherence of dendritic cells is lost …