[HTML][HTML] Monitoring light-induced structural changes of Channelrhodopsin-2 by UV-visible and Fourier transform infrared spectroscopy
E Ritter, K Stehfest, A Berndt, P Hegemann… - Journal of Biological …, 2008 - ASBMB
Channelrhodopsin-2 (ChR2) is a microbial type rhodopsin and a light-gated cation channel
that controls phototaxis in Chlamydomonas. We expressed ChR2 in COS-cells, purified it,
and subsequently investigated this unusual photoreceptor by flash photolysis and UV-visible
and Fourier transform infrared difference spectroscopy. Several transient photoproducts of
the wild type ChR2 were identified, and their kinetics and molecular properties were
compared with those of the ChR2 mutant E90Q. Based on the spectroscopic data we …
that controls phototaxis in Chlamydomonas. We expressed ChR2 in COS-cells, purified it,
and subsequently investigated this unusual photoreceptor by flash photolysis and UV-visible
and Fourier transform infrared difference spectroscopy. Several transient photoproducts of
the wild type ChR2 were identified, and their kinetics and molecular properties were
compared with those of the ChR2 mutant E90Q. Based on the spectroscopic data we …