Autophagy‐deficient mice exhibit the formation of ubiquitin‐inclusions in the liver and brain,
which is not attributed to the dysfunction of the ubiquitin‐proteasome system. Moreover, it is
also clear that a multifunctional protein p62/A170/SQSTM1 (hereafter referred to as p62)
links autophagy and inclusion formation, being one of the key components of the ubiquitin
inclusions. The ubiquitin/p62 inclusions can be detected in the detergent‐insoluble fraction
by western blot analysis, while morphological information can be obtained by …