[HTML][HTML] Chronic TNFα and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis
V Bézaire, A Mairal, R Anesia, C Lefort, D Langin - FEBS letters, 2009 - Elsevier
V Bézaire, A Mairal, R Anesia, C Lefort, D Langin
FEBS letters, 2009•ElsevierWe examined the effects of chronic TNFα and dibutyryl-cAMP (Db-cAMP) pre-treatment on
the lipolytic machinery of human hMADS adipocytes. TNFα decreased adipose triglyceride
lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-
hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN)
protein content. Conversely, Db-cAMP increased ATGL and HSL protein content but
prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In …
the lipolytic machinery of human hMADS adipocytes. TNFα decreased adipose triglyceride
lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-
hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN)
protein content. Conversely, Db-cAMP increased ATGL and HSL protein content but
prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In …
We examined the effects of chronic TNFα and dibutyryl-cAMP (Db-cAMP) pre-treatment on the lipolytic machinery of human hMADS adipocytes. TNFα decreased adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN) protein content. Conversely, Db-cAMP increased ATGL and HSL protein content but prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In forskolin-stimulated conditions, TNFα and Db-cAMP pre-treatment decreased stimulated TG-hydrolase activity and impaired PLIN phosphorylation. Together, this resulted in a severely attenuated response to forskolin-stimulated lipolysis.
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