Deep sequencing in microdissected renal tubules identifies nephron segment–specific transcriptomes

JW Lee, CL Chou, MA Knepper - Journal of the American Society …, 2015 - journals.lww.com
The function of each renal tubule segment depends on the genes expressed therein. High-
throughput methods used for global profiling of gene expression in unique cell types have
shown low sensitivity and high false positivity, thereby limiting the usefulness of these
methods in transcriptomic research. However, deep sequencing of RNA species (RNA-seq)
achieves highly sensitive and quantitative transcriptomic profiling by sequencing RNAs in a
massive, parallel manner. Here, we used RNA-seq coupled with classic renal tubule …