[HTML][HTML] The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical …

T Shinkawa, K Nakamura, N Yamane… - Journal of Biological …, 2003 - ASBMB
An anti-human interleukin 5 receptor (hIL-5R) humanized immunoglobulin G1 (IgG1) and an
anti-CD20 chimeric IgG1 produced by rat hybridoma YB2/0 cell lines showed more than 50-
fold higher antibody-dependent cellular cytotoxicity (ADCC) using purified human peripheral
blood mononuclear cells as effector than those produced by Chinese hamster ovary (CHO)
cell lines. Monosaccharide composition and oligosaccharide profiling analysis showed that
low fucose (Fuc) content of complex-type oligosaccharides was characteristic in YB2/0 …