[HTML][HTML] Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment
JL Dermody, S Buratowski - Journal of Biological Chemistry, 2010 - ASBMB
The Paf1 complex (Paf1C) affects RNA polymerase II transcription by coordinating co-
transcriptional chromatin modifications and helping recruit mRNA 3′ end processing
factors. Paf1C cross-links to transcribed genes, but not downstream of the cleavage and
polyadenylation site, suggesting that it may interact with the nascent mRNA. Paf1C purified
from Saccharomyces cerevisiae binds RNA in vitro, as do the purified Leo1 and Rtf1
subunits of the complex. In vivo cross-linking and immunoprecipitation of RNA associated …
transcriptional chromatin modifications and helping recruit mRNA 3′ end processing
factors. Paf1C cross-links to transcribed genes, but not downstream of the cleavage and
polyadenylation site, suggesting that it may interact with the nascent mRNA. Paf1C purified
from Saccharomyces cerevisiae binds RNA in vitro, as do the purified Leo1 and Rtf1
subunits of the complex. In vivo cross-linking and immunoprecipitation of RNA associated …