Flat cultures of mammalian cells are a widely used in vitro approach for understanding cell
physiology, but this system is limited in modeling solid tissues due to unnaturally rapid cell
replication. This is particularly challenging when modeling mature chromatin, as fast
replicating cells are frequently involved in DNA replication and have a heterogeneous
polyploid population. Presented below is a workflow for modeling, treating, and analyzing
quiescent chromatin modifications using a three-dimensional (3D) cell culture system. Using …